Hank’s Balanced Salt Solution

Hank’s Balanced Salt Solution (HBSS) is a buffered saline solution formulated to mimic the ionic composition of mammalian extracellular fluid. It contains various inorganic salts, such as sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, and sodium phosphate, in concentrations similar to those found in physiological fluids. HBSS is commonly used in cell culture and biological research for washing, maintaining, and storing cells and tissues. It provides a physiologically balanced environment to support cell viability and function during experiments, such as cell isolation, cell washing, and tissue dissection. Additionally, HBSS is utilized in various laboratory techniques, including cell culture assays, immunohistochemistry, and molecular biology applications.